Gene insertion, also known as targeted integration, is a type of gene editing that involves adding a new gene to a specific location in the genome. This is achieved through the use of nucleases, enzymes that cut DNA at specific locations. The cut DNA is then repaired using a DNA template that contains the desired gene. This results in the insertion of the new gene into the genome.

Gene insertion has the potential to revolutionize research with strains. Rather than correcting individual mutations, gene insertion can theoretically correct virtually all mutations in a gene. As a result, it is a highly versatile and powerful tool for developing new drugs based on the genetic component.

Fig. 1. cDNA insertion into the plasmid and the transformation of the E-coli the general steps. (Saifuddin N.M, 2001)