mRNA display is a powerful in vitro selection technology, which allows the selection of peptides with the required functions from the trillions of variant libraries, and can achieve the introduction of unnatural amino acids in the display peptide library to develop peptides that target multiple protein-protein interaction targets.

mRNA Display for Peptide Discovery Services

mRNA display is a selective technology, which is based on the formation of covalent bonds between peptides and their encoded mRNA molecules during in vitro translation. First, the mRNA is modified at its 3’ end with puromycin – an antibiotic that mimics the structure of an amino acylated tRNA. At the end of the process of translating this modified mRNA into a new peptide chain, puromycin enters the A site of the ribosome and forms a peptide bond with the C-terminal of the translated peptide, thereby covalently connecting the mRNA and the peptide. Stable genotype-phenotype linkage allows the protein to be directly amplified, and enables the peptide chain displayed by mRNA to have the required characteristics. If it is to develop peptide-like molecules for studying protein-protein interactions, it is usually to introduce some restrictive structures in the translation process through various strategies to build a restrictive display peptide library.

Fig . 1 mRNA display is used for the construction of peptides. (Dongen D, 2022)

Services

• mRNA Display-based Peptide Library Construction
• mRNA Display-based Peptide Screening
• mRNA Display-based Peptidomimetic Development